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Illumina - HiSeq 2500 V.3 DNA Genome Sequencer

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Illumina HiSeq 2500 V.3 DNA Genome Sequencer



Used - unit is in good working condition, please see photos for details. Includes everything pictured. Does not have a cBot.  Unit will be tested before shipping. Comes with our 30-day, 100% no hassle warranty.


The HiSeq 2500 System is a powerful high-throughput sequencing system. High-quality data using proven Illumina SBS chemistry has made it the instrument of choice for major genome centers and research institutions throughout the world.


Flexibility for Multiple Applications


While the HiSeq 2500 System can generate massive data, it is also highly flexible.


The HiSeq 2500 is the first Illumina sequencing platform to feature two run modes—rapid run and high-output run mode. Furthermore, the system can be configured to run one flow cell alone or two flow cells simultaneously—providing a flexible and scalable platform that supports a broad range of applications and study sizes and enables a solution tailored to your specific research needs.


Rapid-run mode leverages on-board cluster generation and reduced cycle times to significantly accelerate run times. In rapid-run mode, fully automated clustering and sequencing for short read applications can be completed in 7 hours and a single human genome at 30× coverage can be completed in 27 hours.


In high output mode, the HiSeq 2500 System can process 8 human genomes at 30× coverage or 150 human exomes, assuming 4 Gb per exome at 2 × 75 bp, per run.


The HiSeq 2500 System, in high-output mode, is perfect for production scale projects with large genome sizes or studies with hundreds of samples. 


With rapid or high-output modes and single or dual flow cell options, the HiSeq 2500 can be tuned to meet output needs ranging from 10 Gb to 1 Tb or 300 million to 4 billion reads per run, supporting the broadest range of applications.


Choose between rapid run and high output modes to fit your project needs:


  • Rapid Run Mode Produces Quick Results

    Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 250 base pair reads. The longer reads enable greater depth of coverage, improved assembly for de novo applications and aid in long-read applications such as metagenomic analyses.

  • High Output Mode for Larger Studies

    High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process over 6× more samples than rapid mode and to complete large projects quickly and efficiently. HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.


Incredible Speed and Throughput




The HiSeq 2500 System delivers the highest daily throughput and total yield of any individually sold sequencer on the market today. With a massive daily throughput of 160 Gb per day or 1 Tb per run, more samples can be simultaneously sequenced at a greater depth than ever before—producing richer, more meaningful data. Large studies can be completed with reduced hands-on time and reagent cost. 


Exceptional Data Quality


With the HiSeq 2500 System, high data quality is ensured by leveraging the most widely adopted chemistry in the industry: sequencing by synthesis (SBS). SBS technology on the HiSeq 2500 utilizes a proprietary reversible terminator-based method that detects single bases as they are incorporated into DNA template strands. As all four reversible, terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias and greatly reduces raw error rates compared to other technologies.2,3 The end result is highly accurate base-by-base sequencing that virtually eliminates sequence-context-specific errors, even within repetitive sequence regions and homopolymers. Illumina sequencing delivers the most accurate human genome, the highest yield of errorfree reads, and the highest percentage of base calls above Q30 in the industry.


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