Perkin Elmer – Flexar SQ 300 MS/HPLC System


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Perkin Elmer Flexar SQ 300 MS/HPLC System w/Computer, Software & Other Accessories!

Used – unit is fully functional. Great condition, please see photos for details. Includes everything pictured. Unit will be tested before shipping. Comes with our 30-day, 100% no hassle warranty.

Includes: Computer, Monitor, Keyboard, Mouse and Software. 

Other system components included are:

  • Perkin Elmer Flexar SQ 300 MS Detector, Valve & Syringe Pump
  • Edwards E2M28 Dual Stage Rotary Vane Vacuum Pump

  • Gilson 307 Pump

  • Rheodyne MRA for LC/MS

  • Gilson 333/334 Prep-scale HPLC Pumps

  • Gilson UV/VIS-156 Detector

  • Gilson 506C System Interface

  • Gilson GX-271 Liquid Handler

The systems’s wide flow rate range (from the 333 and 334 Pumps) allows for both semi-preparative and preparative separations. High pumping power accommodates a wide range of preparative column sizes. All injection modules feature a continuous flow path design. The system can accommodate large injection volumes with minimal sample waste and carryover. The new rinse station system incorporates a flowing jet wash and allows the use of up to two different rinse solvents.

Perkin Elmer Flexar SQ 300 MS Detector, Valve & Syringe Pump

With the Flexar™ SQ 300 MS, PerkinElmer has designed the ultimate single quadrupole MS detector, a cutting-edge instrument that features the latest ion source technologies.

The design of the Flexar SQ 300 MS can be divided into three distinct areas—ion source, ion transfer and mass analyzer. Each is completely decoupled to deliver complete and efficient control of their specific processes.

Used for Applications in the following Industries:

  • Food/Nutraceutical
  • Pharmaceutical
  • Chemical/Industrial
  • Environmental
  • Clinical

Instrument Capabilities Overview:

  • Mass Range: 20-3,000 u
  • Mass Accuracy: ± 0.1 u/24 hr; ± 3 ºC
  • Mass Axis Stability: ± 0.1 u/24 hr; ± 3 ºC
  • Max Scan Rate: 10,000 u/sec
  • Resolution (FWHM): 0.6 u ± 0.1
  • Polarity Switching: Standard
  • Quantitative Dynamic Range: 105


For additional, more detailed product information on the Flexar SQ 300 MS Detector, please visit the following website:


Edwards E2M28 Dual Stage Rotary Vane Vacuum Pump

The E2M28 pump is a two-stage, direct drive, sliding vane pump. The pump is oil sealed and designed for reliable, long-term operation in both laboratory and industrial environments. The pump is a freestanding unit with the drive provided through a flexible coupling by either a single-phase or three-phase (four-pole) motor. 

The motor is totally enclosed and is cooled by the motor-cooling fan which directs air along the motor fins. The motors are fitted with a thermal overload device. When the motor is too hot, the thermal overload device switches off the pump. The thermal overload device has an automatic reset; when the motor cools down, the device resets and the motor will restart. 
An oil pressure system lubricates the pump shaft bearing surfaces and rotor sealing faces. The pump has an oil distribution valve which prevents discharge of oil to the pump interior (suckback) after the pump stops. The pumping chambers are air-tight, so this arrangement prevents oil suckback unless the gas ballast valve is open. 
You can inspect the level and condition of oil in the oil box reservoir through the oil sight-glass. An oil filler-plug is fitted to the top of the oil box. The pump has two oil drain-plugs to allow either gravity oil drain or pressure oil drain.

Features and Benefits:

  • The pump is designed for reliable, long-term operation.
  • Thermal overload device prevents overheating (single phase only).
  • The pump is a free-standing unit.
  • The drive is provided through a flexible coupling by a single-phase or three-phase (four pole) motor.
  • The motors are totally enclosed and are cooled by the motor-cooling fan.
  • Easy to use controls.
  • Pressure die cast oil box with integral oil seals prevents oil leaks.
  • Gas ballast valve for large water vapour handling capacity.
  • Externally approved to EN61010, CSA, C22.2 and UL61010.
  • Oil distribution valve.
  • Pumping chambers are air-tight.
  • Oil sight-glass, two oil drain-plugs, inlet-port and outlet nozzle all fitted as standard.
  • Gas-ballast control.

Includes EMF20 Oil Mist Filter:

Mist filters capture oil mist from the outlet of pumps, which would otherwise be ejected into the atmosphere. This may happen when you use gas ballast or when you pump high gas throughputs. You can also return oil trapped in the mist filter back to the pump, although you must ensure that the process gases will not contaminate the pump or pump oil. 

The EMF20 mist filter can be used on low pressure applications. They are very efficient at 99.999% DOP test and are also azide proof. The white bottom half of the body is semi-transparent, allowing the oil level to be monitored. The EMF filters have a unique odor element which neutralizes the smell of oil mist. 

“Please note: Due to shipping regulations, all vacuum pumps will be shipped without pump oil. Oiled vacuum pumps SHOULD NOT be run without oil and doing so will VOID the warranty! Please contact us if you would like to include a quart of vacuum pump oil with your order for an additional $15/qt charge.”


Gilson 307 Pump

The Gilson 307 Piston Pump is designed to produce accurate and reproducible flow rates in the range 0.025 to 5 ml/min. It can be programmed to give flow rate gradients and to synchronize with other instruments using the inputs and outputs.

Interchangeable pump heads accommodate flow rates ranging from 5µL/min to 200mL/min with a wide range of liquids at differing viscosities.

Together with Gilson Manometric Modules and Dynamic Mixers, these Pumps offer a cost-effective, robust solution for isocratic to gradient, analytical to preparative HPLC.

Features & Benefits:

  • Designed primarily for research and QC/QA in the isocratic mode, and offers flow rates from 10 µL/min to 10 mL/min
  • Fully programmable through a front panel keypad to provide system control capabilities
  • Smooth flows are enhanced by a built-in pulse dampener
  • Pumps heads are designed for quick and easy maintenance—piston seals, check valves and other parts can be replaced in a matter of minutes
  • Has a built-in Manometric Module
  • Dynamic Mixers ensure efficient, problem-free mobile phase mixing
  • Can be configured for use in a sample prep GPC or GPC/SPE system

For additional, more detailed product information on the Gilson 307 Pump, please visit the following website:


Rheodyne MRA for LC/MS

The Rheodyne MRA100-000 from IDEX Health & Science is an active splitting device that delivers a small aliquot of an HPLC stream into a Mass Spec.

The Mass Rate Attenuator can be used in analytical scale to prep scale flow rate applications with little or no observable post-column band broadening.

The Rheodyne MRA can be operated with Input Line Control or via RS232 communication.

The ability to select from a wide range of split rations will add convenience, productivity and time savings to the LC/MS applications. The Flow Splitter offers 4-ports with a 6-position configuration.

  • 59 discrete LC/MS flow split ratios range from 100:1 to 100,000:1
    • Split ratios will remain unaffected by mobile phase viscosity, tubing length and temperature changes. 


For additional, more detailed product information on the Rheodyne MRA, please visit the following website:


Gilson 333/334 Prep-scale HPLC Pumps

The Gilson 333 and 334 pumps are reciprocating piston pumps for use in preparative chromatography using from 20 to 100 mm bore columns. They are individually known as the 333 Pump and the 334 Pump and are individually for single solvent delivery under high pressure. Together they are described as Gilson 333-334, a gradient system for binary gradient applications.

Each pump is equipped with two pump heads.

Complete preparative pumping solution – isocratic (333 pump) or binary gradient applications.

One preparative pumping solution to accommodate columns ranging from 10 mm to 50 mm ID in high pressure.


For additional, more detailed product information on each pump (333 & 334), please visit the following website:


  • Gilson 333 Pump

Your delivery solution for preparative chromatography. Capable of fast separations with high-efficiency columns in normal and reverse-phase modes. This 333 Master Pump is for isocratic applications. 

333 Pumps have a control panel (integral keypad and screen) for configuring, programming, and coordinating with other equipment (pumps, detectors, injectors, etc.).

Features & Benefits:

  • Milligram-to-gram level sample mass throughput per injection; up to 15 g depending on column size and loading capacity
  • Ability to operate additional pumps in parallel to double the flow rate up to 400 mL/min
  • Control from 333 master pump: flow-rate gradient, four-solvent selection and step gradient (additional valve), composition gradient (one or two additional pumps), sample-injection pump, UV/VIS detector and electrical input/output contacts with any other associated equipment
  • Use the control panel (integral keypad and screen) for configuring, programming, and coordinating with other equipment (pumps, detectors, injectors, etc.)
  • Designed to pump up to three solvents (depending on the configuration). They also have an integrated pressure, purge and mixing module (PPMM), an outlet filter and optionally can house a solvent valve block
  • Quiet operation, durable design
  • Stackable to conserve bench space


  • Gilson 334 Pump

334 Pumps are designed as slave pumps for delivery of a second solvent (B) and (with two additional 334 Pumps) a third solvent (C), to be mixed with the primary solvent (A) pumped by the 333.

334 Pumps have no PPMM, no outlet filter, and because they have no control panel they are physically shorter. They may be controlled by 333 Pump software using its control panel.


Gilson UV/VIS-156 Detector

The 156 UV/VIS Detector is a microprocessor-controlled, versatile ultraviolet-visible absorption detector that provides dual wavelength detection.

The detector has a sensitivity range of 0.001 to 2.0 AUFS (Absorbance Units, Full Scale).


You can choose from the following modes to specify detection conditions and basic operating parameters:

  • single wavelength
  • dual wavelength
  • scan wavelength
  • setup
  • status
  • file

Within a mode, you set parameters to optimize sample detection using a wavelength between 190 and 700 nm. You can also program the detector to choose new parameters at any preset time during a run.


For additional, more detailed product information on the UV/VIS-156 Detector, please visit the following website:


Gilson 506C System Interface

The Gilson 506C System Interface connects your computer to many Gilson Instruments. It permits bi-directional communication by interconverting the RS-232 signal levels used by computers and RS-422/485 signal levels required by the Gilson Serial Input/Output Channel (GSIOC).

  • Provides four contact inputs and six contact outputs that allow your computer to control instruments not equipped for GSIOC operation
  • The state of the contacts can be read or changed using appropriate software (such as Gilson control software)
  • The system interface incorporates four analog data inputs that can be read by the computer via the GSIOC
  • Using these data inputs, your computer can read the signal from any detector or monitor that provides a –100 mV to +1.0 V analog input
  • Includes a socket for installing the security key required to operate most Gilson software products


Gilson GX-271 Liquid Handler

The GX-271 Liquid Handler provides a small footprint, medium capacity liquid handler and autosampler offering small to large volume injection, fraction collection and liquid handling capabilities.

This instrument can be configured with either a GX Prep (included) or Analytical Solvent System which features a syringeless pumping technology capable of delivering a wide range of flow rates and volumes.

Taking up only 23” of linear bench space with a mid-size bed capacity, the GX-271 is the perfect blend of size, performance and capacity.

All injection modules feature a continuous flow path design for preparative flow rates up to 200 mL/min. The GX-271 can accommodate large injection volumes up to 25 mL with minimal sample waste and carryover.

  • The GX-271 Liquid Handler or autosampler can be configured with a GX Direct Injection Module (included) for injection onto an HPLC or LC/MS system. The GX Direct Injection Module features an innovative injection port design that attaches directly to the injection valve reducing void volumes and minimizing carryover associated with calibrated connection tubing.
  • Jet Wash Rinse technology features a flowing rinse for fast and efficient rinsing protocols. The GX-271 is capable of utilizing two separate flowing rinse solvents.
  • Modular design allows the use of Code 20, 200 or 34X Series Racks.
  • A variety of vessels can be accommodated including tubes, vials and microplates in various of formats.
  • Volume capabilities ranging from 50 µL to hundreds of milliliters.
  • Flow rates up to 50 mL/min.
  • The GX Direct Injection Module offers sample loop sizes ranging from 2 µL to 25 mL providing the largest sample volume range on the market today.
  • On-bed reservoirs allow access to four solvents without utilizing a position on the bed.

Additional information

Weight 450 lbs
Dimensions 55 x 60 x 50 in